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rDNA ITS based identification of Eukaryotes and their communication via DOIs
Curation of DNA sequences

Third-party annotations are a valuable resource to improve the quality of public DNA sequences. For example, sequences in International Nucleotide Sequence Databases Collaboration (INSDC) often lack important features like taxon interactions, species level identification, information associated with material source, habitat, country, geolocation, etc.

PlutoF offers a third-party curation service to improve the quality of public DNA sequences and their source metadata (read the related ARPHA Preprint in Research Ideas and Outcomes). UNITE Community is using these services to annotate and improve the quality of INSDC rDNA Internal Transcribed Spacer (ITS) sequence datasets. In collaboration with EMBL-EBI, improved or corrected annotations on INSD sequences in PlutoF are fed back to primary repositories through operating the ELIXIR Contextual Data ClearingHouse (CDCH).

Searching and browsing of third-party annotations introduced by the UNITE Community can be done via ELIXIR CDCH RESTful API or using search interfaces of PlutoF and UNITE.


Curations by the UNITE community members

Curations by the UNITE community members

Number of taxonomic re-annotations 241 469
Number specifications of EcM lineage 256 663
Number of specifications of country of collection 115 868
Number of specifications of host and interacting taxa 55 140
Number of chimeras excluded 4 082
Number of low-quality sequences excluded 9 159
Number of sequences with specimen/culture metadata annotated 10 619
Number of sequences linked to type specimen/culture 4 030
Number of sequences flagged with type status 35 578

Curations sent to EMBL-EBI / ENA

Number of taxonomic re-annotations2 565
Number of specifications of location6 645
Number of specifications of type status289

Browse curations

Third-party annotations can be searched and browsed on UNITE Search Pages